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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in several tumors, which ends up in chromosome instability, and thus may perhaps contribute to tumorigenesis. This protein was found to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a important position in marketing cell-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are already described. Two added splice variants had been described but detailed nucleotide sequence details just isn't but accessible.
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Blocking peptides are peptides that bind especially to your concentrate on antibody and block antibody binding. These peptide ordinarily consists of the epitope acknowledged through the antibody.
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This guideline outlines even further doable optimization of mobile and immunoassay parameters to make certain the best possible results are...
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This information outlines even further attainable optimization of cellular and immunoassay parameters to make sure the very best results are...
Antibodies bound to the blocking peptide no more bind on the epitope to the focus on protein. By evaluating the staining in the blocked antibody vs . the antibody by itself, one can see which staining is precise.
Concentrate on Info Cyclin E belongs towards the really conserved cyclin loved ones, whose users are characterized by a extraordinary periodicity in protein abundance throughout the mobile cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins show distinct expression and degradation styles which add on the temporal coordination of each mitotic function. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S transition. This protein accumulates within the G1-S period boundary which is degraded as cells development by means of S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...
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